三分排列3

tRNA堿基修飾檢測

  • 簡介
  • 分析種類
  • 結果展示
  • Aksomics(康成生物)的質譜檢測平臺能夠為客戶提供全面而準確的tRNA修飾譜。利用Agilent質譜儀,檢測36種修飾堿基在各個樣品中的修飾豐度。


    服務特色:

    ● 優化的前期處理與Agilent優質設備 

    ● 全面而準確的堿基修飾分析


    分析流程:

    圖釋:LC-MS tRNA修飾檢測服務流程圖。


  • Number Nucleoside Symbol Number Nucleoside Symbol
    01 3′-O-methyladenosine 3′-OMeA 19 1-methylguanosine m1G
    02 2′-O-methylcytidine Cm 20 7-methylguanosine m7G
    03 5-methylcytidine m5C 21 N2-methylguanosine m2G
    04 N6-isopentenyladenosine i6A 22 3′-O-methylinosine 3′-OMeI
    05 5,2′-O-dimethylcytidine m5Cm 23 2-thiouridine s2U
    06 1-methyladenosine m1A 24 4-thiouridine s4U
    07 2-thiocytidine s2C 25 5-methyluridine m5U
    08 N2,N2,7-trimethylguanosine m2,2,7G 26 N4-acetylcytidine ac4C
    09 N4-acetyl-2′-O-methylcytidine ac4Cm 27 3′-O-methyluridine 3′-OMeU
    10 N6-methyladenosine m6A 28 5-methyl-2-thiouridine m5s2U
    11 3-methylcytidine m3C 29 5-methoxyuridine mo5U
    12 3′-O-methylcytidine 3′-OMeC 30 pseudouridine Ψ
    13 2′-O-methyladenosine Am 31 2′-O-methylinosine Im
    14 N2,N2-dimethylguanosine m22G 32 3-methyluridine m3U
    15 5′-O-methylthymidine 5′-OMeT 33 3′-O-methylguanosine 3′-OMeG
    16 2′-O-methyluridine Um 34 1-methylpseudouridine m1Ψ
    17 inosine I 35 5-hydroxymethylcytidine hm5C
    18 2′-O-methylguanosine Gm 36 5,2′-O-dimethyluridine m5Um
  • 1. 修飾定量分析

    圖釋:tRNA修飾數量分析圖。


    2. 總離子流程圖

    圖釋:總離子流程圖